Phosvitin phosphate content. Implications for protein kinase assay

Khalil Ahmed, Michael J. Wilson, Alan T. Davis

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The maximal rates of the protein kinase (ATP: protein phosphotransferase, EC 2.7.1.37) reaction studied with chicken egg yolk phosvitin as substrate are dependent on the level of dephosphorylation of phosvitin. 30% dephospho-phosvitin gives the optimal initial rates. With varying levels of dephosphorylation, the apparent Km for the substrate also changes in a biphasic manner. If this factor is taken into account, and a suitable adjustment is made for the concentration of dephospho-phosvitin in the reaction, it is possible to achieve maximal rates for the kinase reaction with phosvitin preparations of varying levels of dephosphorylation. Such a consideration is important for comparing the results of protein kinase studies using phosvitin as the substrate.

Original languageEnglish (US)
Pages (from-to)80-83
Number of pages4
JournalBBA - Enzymology
Volume377
Issue number1
DOIs
StatePublished - Jan 23 1975

Fingerprint Dive into the research topics of 'Phosvitin phosphate content. Implications for protein kinase assay'. Together they form a unique fingerprint.

  • Cite this