Phosphorylation Regulates Integration of the Yeast Ty5 Retrotransposon into Heterochromatin

Junbiao Dai, Weiwu Xie, Troy L. Brady, Jiquan Gao, Daniel F. Voytas

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The yeast Ty5 retrotransposon preferentially integrates into heterochromatin at the telomeres and silent mating loci. Target specificity is mediated by a small domain of Ty5 integrase (the targeting domain, TD), which interacts with the heterochromatin protein Sir4 and tethers the integration complex to target sites. Here we demonstrate that TD is phosphorylated and that phosphorylation is required for interaction with Sir4. The yeast cell, therefore, through posttranslational modification, controls Ty5's mutagenic potential: when TD is phosphorylated, insertions occur in gene-poor heterochromatin, thereby minimizing deleterious consequences of transposition; however, in the absence of phosphorylation, Ty5 integrates throughout the genome, frequently causing mutations. TD phosphorylation is reduced under stress conditions, specifically starvation for amino acids, nitrogen, or fermentable carbon. This suggests that Ty5 target specificity changes in response to nutrient availability and is consistent with McClintock's hypothesis that mobile elements restructure host genomes as an adaptive response to environmental challenge.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalMolecular Cell
Volume27
Issue number2
DOIs
StatePublished - Jul 20 2007

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Retroelements
Heterochromatin
Yeasts
Phosphorylation
Genome
Integrases
Telomere
Post Translational Protein Processing
Starvation
Nitrogen
Carbon
Amino Acids
Food
Mutation
Genes
Proteins

Keywords

  • DNA

Cite this

Phosphorylation Regulates Integration of the Yeast Ty5 Retrotransposon into Heterochromatin. / Dai, Junbiao; Xie, Weiwu; Brady, Troy L.; Gao, Jiquan; Voytas, Daniel F.

In: Molecular Cell, Vol. 27, No. 2, 20.07.2007, p. 289-299.

Research output: Contribution to journalArticle

Dai, Junbiao ; Xie, Weiwu ; Brady, Troy L. ; Gao, Jiquan ; Voytas, Daniel F. / Phosphorylation Regulates Integration of the Yeast Ty5 Retrotransposon into Heterochromatin. In: Molecular Cell. 2007 ; Vol. 27, No. 2. pp. 289-299.
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