Phosphohistone and glycogen synthase D phosphatase activities in a liver glycogen pellet preparation.

D. P. Gilboe, frank q Nuttall

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A liver glycogen pellet preparation previously found to contain synthase D phosphatase activity was shown to contain also phosphohistone phosphatase activity. Pellet phosphohistone phosphatase and synthase D phosphatase competed for the same substrates and appeared to be the same enzyme. ATP, a potent inhibitor, and G-6-P, a potent activator of the synthase phosphatase reaction, had little effect on the phosphohistone phosphatase reaction. These observations suggest that the ATP and G-6-P effects are relatively specific and are probably caused by binding to the synthase D substrate. The observed effects of NaCl and KCl were more complex. They stimulated phosphohistone phosphatase activity but strikingly inhibited synthase phosphatase activity. Sodium fluoride inhibited both reactions.

Original languageEnglish (US)
Pages (from-to)405-415
Number of pages11
JournalPhysiological chemistry and physics
Volume8
Issue number5
StatePublished - Dec 1 1976
Externally publishedYes

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