Phorbol ester-induced changes in human leukemic pre-B lines

Nicoletta Sacchi, Tucker V. LeBien, Stephen Trost, Diego Breviario, F. J. Bollum

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalCellular Immunology
Volume84
Issue number1
DOIs
StatePublished - Mar 1984

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