Abstract
The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.
Original language | English (US) |
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Pages (from-to) | 65-73 |
Number of pages | 9 |
Journal | Cellular Immunology |
Volume | 84 |
Issue number | 1 |
DOIs | |
State | Published - Mar 1984 |
Externally published | Yes |