Phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not Alter the Effects of Bacterial and Amplified Plaque Extracts on Cultures of Fibroblasts from Normal and Overgrown Gingivae

Q. T. Smith, James E Hinrichs

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and “amplified” plaque extracts, and (c) microbial and “amplified” plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.

Original languageEnglish (US)
Pages (from-to)1393-1398
Number of pages6
JournalJournal of dental research
Volume66
Issue number8
DOIs
StatePublished - Jan 1 1987

Fingerprint

Phenytoin
Gingiva
Fibroblasts
Gingival Overgrowth
Cell Count
Drug Interactions
5-phenylhydantoin
Proteins
Therapeutics

Cite this

@article{28394ad738b940baa73545d18d72f0a9,
title = "Phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not Alter the Effects of Bacterial and Amplified Plaque Extracts on Cultures of Fibroblasts from Normal and Overgrown Gingivae",
abstract = "Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and “amplified” plaque extracts, and (c) microbial and “amplified” plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.",
author = "Smith, {Q. T.} and Hinrichs, {James E}",
year = "1987",
month = "1",
day = "1",
doi = "10.1177/00220345870660082201",
language = "English (US)",
volume = "66",
pages = "1393--1398",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "8",

}

TY - JOUR

T1 - Phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not Alter the Effects of Bacterial and Amplified Plaque Extracts on Cultures of Fibroblasts from Normal and Overgrown Gingivae

AU - Smith, Q. T.

AU - Hinrichs, James E

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and “amplified” plaque extracts, and (c) microbial and “amplified” plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.

AB - Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and “amplified” plaque extracts, and (c) microbial and “amplified” plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.

UR - http://www.scopus.com/inward/record.url?scp=0023196775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023196775&partnerID=8YFLogxK

U2 - 10.1177/00220345870660082201

DO - 10.1177/00220345870660082201

M3 - Article

VL - 66

SP - 1393

EP - 1398

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 8

ER -