Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

Nymul Khan, Yukari Maezato, Ryan S. McClure, Colin J. Brislawn, Jennifer M. Mobberley, Nancy Isern, William B. Chrisler, Lye Meng Markillie, Brett M. Barney, Hyun Seob Song, William C. Nelson, Hans C. Bernstein

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized - and confirmed - that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.

Original languageEnglish (US)
Article number297
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Symbiosis
Coculture Techniques
Marinobacter
Halomonas
Growth
Phenotype
Microbial Interactions
Glucose
Controlled Environment
Xylose
Carbon
Bacteria
Food

Cite this

Khan, N., Maezato, Y., McClure, R. S., Brislawn, C. J., Mobberley, J. M., Isern, N., ... Bernstein, H. C. (2018). Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture. Scientific reports, 8(1), [297]. https://doi.org/10.1038/s41598-017-18630-1

Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture. / Khan, Nymul; Maezato, Yukari; McClure, Ryan S.; Brislawn, Colin J.; Mobberley, Jennifer M.; Isern, Nancy; Chrisler, William B.; Markillie, Lye Meng; Barney, Brett M.; Song, Hyun Seob; Nelson, William C.; Bernstein, Hans C.

In: Scientific reports, Vol. 8, No. 1, 297, 01.12.2018.

Research output: Contribution to journalArticle

Khan, N, Maezato, Y, McClure, RS, Brislawn, CJ, Mobberley, JM, Isern, N, Chrisler, WB, Markillie, LM, Barney, BM, Song, HS, Nelson, WC & Bernstein, HC 2018, 'Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture', Scientific reports, vol. 8, no. 1, 297. https://doi.org/10.1038/s41598-017-18630-1
Khan, Nymul ; Maezato, Yukari ; McClure, Ryan S. ; Brislawn, Colin J. ; Mobberley, Jennifer M. ; Isern, Nancy ; Chrisler, William B. ; Markillie, Lye Meng ; Barney, Brett M. ; Song, Hyun Seob ; Nelson, William C. ; Bernstein, Hans C. / Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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