Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated "Brachyspira hampsonii"

Yogesh Chander, Alexander E Primus, Simone Oliveira, Connie J Gebhart

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States and Canada. Though the primary causative agent of swine dysentery is Brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic Brachyspira sp. This novel Brachyspira sp. was identified from clinical submissions at the Minnesota Veterinary Diagnostic Laboratory, and 40 of such isolates were obtained from 22 clinical submissions representing 5 states. Isolates were confirmed to be different from any known Brachyspira sp. on the basis of phylogenetic analysis of nucleotide sequences of nox and 16S ribosomal RNA (rRNA) genes. Phylogenetic analyses grouped all isolates into 2 clades (clades I and II), and grouping patterns were similar for both nox and 16S rRNA gene sequence analyses. Phenotypically, all isolates were indole and hippurate negative, and enzymatic profiling indicated 2 types of profiles, irrespective of the phylogenetic grouping, differing only in the production of β-glucosidase. The results suggest that a potentially virulent new species of Brachyspira sp., provisionally named "Brachyspira hampsonii ", is circulating among swine herds in the United States.

Original languageEnglish (US)
Pages (from-to)903-910
Number of pages8
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number5
DOIs
StatePublished - Sep 1 2012

Fingerprint

Brachyspira
phylogeny
Swine
Brachyspira hyodysenteriae
16S Ribosomal RNA
Glucosidases
swine dysentery
RNA Sequence Analysis
nucleotide sequences
Dysentery
glucosidases
colitis
indoles
Colitis
rRNA Genes
Canada
Disease Outbreaks
Diarrhea
diarrhea
herds

Keywords

  • 16S ribosomal RNA
  • Brachyspira hampsonii
  • Brachyspira sp
  • nox gene
  • phenotype
  • swine dysentery

Cite this

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abstract = "Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States and Canada. Though the primary causative agent of swine dysentery is Brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic Brachyspira sp. This novel Brachyspira sp. was identified from clinical submissions at the Minnesota Veterinary Diagnostic Laboratory, and 40 of such isolates were obtained from 22 clinical submissions representing 5 states. Isolates were confirmed to be different from any known Brachyspira sp. on the basis of phylogenetic analysis of nucleotide sequences of nox and 16S ribosomal RNA (rRNA) genes. Phylogenetic analyses grouped all isolates into 2 clades (clades I and II), and grouping patterns were similar for both nox and 16S rRNA gene sequence analyses. Phenotypically, all isolates were indole and hippurate negative, and enzymatic profiling indicated 2 types of profiles, irrespective of the phylogenetic grouping, differing only in the production of β-glucosidase. The results suggest that a potentially virulent new species of Brachyspira sp., provisionally named {"}Brachyspira hampsonii {"}, is circulating among swine herds in the United States.",
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