Phase diagrams of lipid mixtures relevant to the study of membrane rafts

Félix M. Goñi; Alicia Alonso; Luis A. Bagatolli; Rhoderick E. Brown; Derek Marsh; Manuel Prieto; Jenifer L. Thewalt

doi:10.1016/j.bbalip.2008.09.002

Phase diagrams of lipid mixtures relevant to the study of membrane rafts

Félix M. Goñi, Alicia Alonso, Luis A. Bagatolli, Rhoderick E. Brown, Derek Marsh, Manuel Prieto, Jenifer L. Thewalt

The Hormel Institute

Research output: Contribution to journal › Review article › peer-review

184 Scopus citations

Abstract

The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, and detergent solubilization assays, are critically evaluated and rationalized in terms of triangular phase diagrams. The remaining uncertainties are discussed specifically and separately from the data on which a consensus appears to exist.

Original language	English (US)
Pages (from-to)	665-684
Number of pages	20
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1781
Issue number	11-12
DOIs	https://doi.org/10.1016/j.bbalip.2008.09.002
State	Published - Nov 2008

Bibliographical note

Funding Information:
F.M.G. and A.A. thank the Spanish Ministerio de Educación y Ciencia (grant No. BFU 2007-62062 and No. BFU 2008-01637) and the Basque Government (grant No. IT-461-07) for their support. Research in the laboratory of L.A.B. is funded by a grant from FNU and FSS, Denmark and by the Danish National Research Foundation (which supports the MEMPHYS-Center for Biomembrane Physics). R.E.B. gratefully acknowledges the continuing financial support provided by NIH/NIGMS GM45928 and by the Hormel Foundation. M.P. acknowledges support from F.C.T. (Portugal). J.T. thanks the Natural Sciences and Engineering Research Council of Canada.

Keywords

Cholesterol
Lipid phase diagrams
Membrane rafts
Phosphatidylcholine
Sphingomyelin

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10.1016/j.bbalip.2008.09.002

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title = "Phase diagrams of lipid mixtures relevant to the study of membrane rafts",

abstract = "The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane {"}raft{"} microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, and detergent solubilization assays, are critically evaluated and rationalized in terms of triangular phase diagrams. The remaining uncertainties are discussed specifically and separately from the data on which a consensus appears to exist.",

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note = "Funding Information: F.M.G. and A.A. thank the Spanish Ministerio de Educaci{\'o}n y Ciencia (grant No. BFU 2007-62062 and No. BFU 2008-01637) and the Basque Government (grant No. IT-461-07) for their support. Research in the laboratory of L.A.B. is funded by a grant from FNU and FSS, Denmark and by the Danish National Research Foundation (which supports the MEMPHYS-Center for Biomembrane Physics). R.E.B. gratefully acknowledges the continuing financial support provided by NIH/NIGMS GM45928 and by the Hormel Foundation. M.P. acknowledges support from F.C.T. (Portugal). J.T. thanks the Natural Sciences and Engineering Research Council of Canada.",

year = "2008",

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AU - Goñi, Félix M.

AU - Alonso, Alicia

AU - Bagatolli, Luis A.

AU - Brown, Rhoderick E.

AU - Marsh, Derek

AU - Prieto, Manuel

AU - Thewalt, Jenifer L.

N1 - Funding Information: F.M.G. and A.A. thank the Spanish Ministerio de Educación y Ciencia (grant No. BFU 2007-62062 and No. BFU 2008-01637) and the Basque Government (grant No. IT-461-07) for their support. Research in the laboratory of L.A.B. is funded by a grant from FNU and FSS, Denmark and by the Danish National Research Foundation (which supports the MEMPHYS-Center for Biomembrane Physics). R.E.B. gratefully acknowledges the continuing financial support provided by NIH/NIGMS GM45928 and by the Hormel Foundation. M.P. acknowledges support from F.C.T. (Portugal). J.T. thanks the Natural Sciences and Engineering Research Council of Canada.

PY - 2008/11

Y1 - 2008/11

N2 - The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, and detergent solubilization assays, are critically evaluated and rationalized in terms of triangular phase diagrams. The remaining uncertainties are discussed specifically and separately from the data on which a consensus appears to exist.

AB - The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, and detergent solubilization assays, are critically evaluated and rationalized in terms of triangular phase diagrams. The remaining uncertainties are discussed specifically and separately from the data on which a consensus appears to exist.

KW - Cholesterol

KW - Lipid phase diagrams

KW - Membrane rafts

KW - Phosphatidylcholine

KW - Sphingomyelin

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JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

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IS - 11-12

ER -

Phase diagrams of lipid mixtures relevant to the study of membrane rafts

Abstract

Bibliographical note

Keywords

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