TY - GEN
T1 - Phase change behavior of biomedically relevant solutions
AU - Han, Bumsoo
AU - Devireddy, Ramachandra V.
AU - Bischof, John C
PY - 2002
Y1 - 2002
N2 - Understanding the phase change behavior of biomedically relevant solutions is critical to the development of cryopreservation and cryosurgery protocols. In the present study, the phase change characteristics, particularly thermodynamically non-equilibrium phenomena including supercooled ice nucleation and eutectic formation, of various compositions and concentrations of biological solutions with/without glycerol as a cryoprotective agent (CPA) were investigated using a differential scanning calorimeter (DSC) and cryomicroscopy. The eutectic transitions (crystallization and melting) were observed in not only water-NaCl binary solutions, but also phosphate buffered saline (PBS) solutions. The eutectic transitions in both water-NaCl and PBS solutions were depressed during freezing far below their thermodynamic equilibrium eutectic temperatures. Contrary to freezing, eutectic melting starts very close to the thermodynamic equilibrium eutectic temperature during thawing. However, the eutectic transitions disappeared when a small amount of glycerol (∼0.1M) was added.
AB - Understanding the phase change behavior of biomedically relevant solutions is critical to the development of cryopreservation and cryosurgery protocols. In the present study, the phase change characteristics, particularly thermodynamically non-equilibrium phenomena including supercooled ice nucleation and eutectic formation, of various compositions and concentrations of biological solutions with/without glycerol as a cryoprotective agent (CPA) were investigated using a differential scanning calorimeter (DSC) and cryomicroscopy. The eutectic transitions (crystallization and melting) were observed in not only water-NaCl binary solutions, but also phosphate buffered saline (PBS) solutions. The eutectic transitions in both water-NaCl and PBS solutions were depressed during freezing far below their thermodynamic equilibrium eutectic temperatures. Contrary to freezing, eutectic melting starts very close to the thermodynamic equilibrium eutectic temperature during thawing. However, the eutectic transitions disappeared when a small amount of glycerol (∼0.1M) was added.
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U2 - 10.1115/IMECE2002-32549
DO - 10.1115/IMECE2002-32549
M3 - Conference contribution
AN - SCOPUS:78249271043
SN - 0791836509
SN - 9780791836507
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings
SP - 67
EP - 75
BT - Advances in Bioengineering
PB - American Society of Mechanical Engineers (ASME)
ER -