PH-responsive drug delivery by amphiphilic copolymer through boronate-catechol complexation

Suhong Wu, Ruogu Qi, Huihui Kuang, Yen Wei, Xiabin Jing, Fanbo Meng, Yubin Huang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Stimuli-responsive micellar nanoparticles are gaining considerable attention in the field of drug delivery because of their great advantages of high efficiency in tumor accumulation through the enhanced permeability and retention effect and rapid drug release. In this study, a novel catechol moiety-containing amphiphilic, biodegradable polymeric carrier, monomethoxy poly(ethylene glycol)-block-poly(ε-caprolactone)-block-poly(L-lysine)- graft-[3-(3,4-dihydroxyphenyl)propionic acid], was employed for delivery of the anticancer drug bortezomib (BTZ) to cancer cells. The strategy is based on the facile conjugation of BTZ to catechol-containing polymeric carriers through boronate formation, which could dissociate in acidic environments to liberate its payload BTZ to inhibit proteasome function. This reducing BTZ-catechol interaction with decreasing pHvalue was demonstrated by microcalorimetry analysis using isothermal titration calorimetry. Notably, the obtained micellar BTZ complex that self-assembled from drug-loaded amphiphilic polymer was internalized effectively by MCF-7 breast cancer cells and resulted in significant 26Sproteasome inhibition. Furthermore, the micellar BTZ complex induced remarkable apoptosis on MCF-7 and HeLa cancer cells and exhibited little toxicity on HEK293 normal cells. More importantly, systemic delivery of micellar BTZ complex prolonged its blood circulation and resulted in significant accumulation in the tumor site relative to free drug, thus suggesting therapeutic promise for micellar BTZ delivery in cancer therapy. Trigger happy: A pH-triggered drug-delivery system based on the pH-responsive interaction between the drug bortezomib (BTZ) and a catechol moiety-containing amphiphilic, biodegradable copolymer has been constructed (see scheme; DHPPA=3-(3,4- dihydroxyphenyl)propionic acid). The drug-loaded nanomicelles (M(DB)) not only exhibit damage to cancer cells rather than normal cells invitro, but also accumulate at tumor sites invivo.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalChemPlusChem
Volume78
Issue number2
DOIs
StatePublished - Feb 1 2013

Keywords

  • antitumor agents
  • block copolymers
  • drug delivery
  • nanoparticles
  • pH response

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