Phenylphosphonate was used as an internal reference for 31P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.
|Original language||English (US)|
|Number of pages||4|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 14 1986|
Bibliographical noteFunding Information:
This research was supported by grants ES03270-02, HL33600, and 1K04 HL 01241 from the National Institutes of Health.