pH measurements by 31P NMR in bacterial suspensions using phenyl phosphonate as a probe

W. J. Thoma, J. G. Steiert, R. L. Crawford, K. Ugurbil

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Phenylphosphonate was used as an internal reference for 31P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.

Original languageEnglish (US)
Pages (from-to)1106-1109
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number3
DOIs
StatePublished - Aug 14 1986
Externally publishedYes

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