pH measurements by 31P NMR in bacterial suspensions using phenyl phosphonate as a probe

W. J. Thoma; J. G. Steiert; R. L. Crawford; K. Ugurbil

doi:10.1016/S0006-291X(86)80396-5

pH measurements by ³¹P NMR in bacterial suspensions using phenyl phosphonate as a probe

W. J. Thoma, J. G. Steiert, R. L. Crawford, K. Ugurbil

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Phenylphosphonate was used as an internal reference for ³¹P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.

Original language	English (US)
Pages (from-to)	1106-1109
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	138
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(86)80396-5
State	Published - Aug 14 1986
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by grants ES03270-02, HL33600, and 1K04 HL 01241 from the National Institutes of Health.

Publisher link

10.1016/S0006-291X(86)80396-5

Find It

Find It at U of M Libraries

Cite this

@article{d5389883249e421ea113032e559515a2,

title = "pH measurements by 31P NMR in bacterial suspensions using phenyl phosphonate as a probe",

abstract = "Phenylphosphonate was used as an internal reference for 31P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.",

author = "Thoma, {W. J.} and Steiert, {J. G.} and Crawford, {R. L.} and K. Ugurbil",

note = "Funding Information: This research was supported by grants ES03270-02, HL33600, and 1K04 HL 01241 from the National Institutes of Health.",

year = "1986",

month = aug,

day = "14",

doi = "10.1016/S0006-291X(86)80396-5",

language = "English (US)",

volume = "138",

pages = "1106--1109",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - pH measurements by 31P NMR in bacterial suspensions using phenyl phosphonate as a probe

AU - Thoma, W. J.

AU - Steiert, J. G.

AU - Crawford, R. L.

AU - Ugurbil, K.

N1 - Funding Information: This research was supported by grants ES03270-02, HL33600, and 1K04 HL 01241 from the National Institutes of Health.

PY - 1986/8/14

Y1 - 1986/8/14

N2 - Phenylphosphonate was used as an internal reference for 31P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.

AB - Phenylphosphonate was used as an internal reference for 31P NMR measurements of E. coli cytoplasmic pH. Phenylphosphonate diffused into the cells allowing determination of pH, independent of magnetic susceptability differences between intercellular and extracellular pools. Phenylphosphonate was used to measure pH changes during succinate uptake and metabolism, as well as during uncoupling of the transmembrane pH gradient by pentachlorophenol.

UR - http://www.scopus.com/inward/record.url?scp=0022556796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022556796&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(86)80396-5

DO - 10.1016/S0006-291X(86)80396-5

M3 - Article

C2 - 3019333

AN - SCOPUS:0022556796

SN - 0006-291X

VL - 138

SP - 1106

EP - 1109

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -