Pertussis toxin treatment modifies opiate action in the rat brain striatum

M. E. Abood; P. Y. Law; H. H. Loh

doi:10.1016/S0006-291X(85)80185-6

Pertussis toxin treatment modifies opiate action in the rat brain striatum

M. E. Abood, P. Y. Law, H. H. Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

In this report we present evidence that a guanine nucleotide regulatory protien, G_i, mediates opiate action in the rat brain striatum. Opiates inhibit basal adenylate cyclase activity in rat brain striatum. This effect on adenylate cyclase is dose-dependently attenuated by pretreatment of membranes with pertussis toxin, which ADP-ribosylates a protein with a molecular mass of 41,000 daltons. This protein co-migrates with the GTP-binding subunit of G_i, which mediates inhibition of adenylate cyclase. Several brain regions were compared for the extent of radiolabeling and effects on adenylate cyclase activity. Although G_i was found in each region examined, opiate inhibition of adenylate cyclase is clearly seen only in the striatum.

Original language	English (US)
Pages (from-to)	477-483
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	127
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(85)80185-6
State	Published - Mar 15 1985

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Research Support, U.S. Gov't, P.H.S.

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abstract = "In this report we present evidence that a guanine nucleotide regulatory protien, Gi, mediates opiate action in the rat brain striatum. Opiates inhibit basal adenylate cyclase activity in rat brain striatum. This effect on adenylate cyclase is dose-dependently attenuated by pretreatment of membranes with pertussis toxin, which ADP-ribosylates a protein with a molecular mass of 41,000 daltons. This protein co-migrates with the GTP-binding subunit of Gi, which mediates inhibition of adenylate cyclase. Several brain regions were compared for the extent of radiolabeling and effects on adenylate cyclase activity. Although Gi was found in each region examined, opiate inhibition of adenylate cyclase is clearly seen only in the striatum.",

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AU - Abood, M. E.

AU - Law, P. Y.

AU - Loh, H. H.

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N2 - In this report we present evidence that a guanine nucleotide regulatory protien, Gi, mediates opiate action in the rat brain striatum. Opiates inhibit basal adenylate cyclase activity in rat brain striatum. This effect on adenylate cyclase is dose-dependently attenuated by pretreatment of membranes with pertussis toxin, which ADP-ribosylates a protein with a molecular mass of 41,000 daltons. This protein co-migrates with the GTP-binding subunit of Gi, which mediates inhibition of adenylate cyclase. Several brain regions were compared for the extent of radiolabeling and effects on adenylate cyclase activity. Although Gi was found in each region examined, opiate inhibition of adenylate cyclase is clearly seen only in the striatum.

AB - In this report we present evidence that a guanine nucleotide regulatory protien, Gi, mediates opiate action in the rat brain striatum. Opiates inhibit basal adenylate cyclase activity in rat brain striatum. This effect on adenylate cyclase is dose-dependently attenuated by pretreatment of membranes with pertussis toxin, which ADP-ribosylates a protein with a molecular mass of 41,000 daltons. This protein co-migrates with the GTP-binding subunit of Gi, which mediates inhibition of adenylate cyclase. Several brain regions were compared for the extent of radiolabeling and effects on adenylate cyclase activity. Although Gi was found in each region examined, opiate inhibition of adenylate cyclase is clearly seen only in the striatum.

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Pertussis toxin treatment modifies opiate action in the rat brain striatum

Abstract

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