Pertussis toxin (PTX) induces activation of L-arginine transport in pulmonary artery endothelial cells (PAEC). The effects of PTX on L-arginine transport appeared after 6 h of treatment and reached maximal values after treatment for 12 h. PTX-induced changes in L-arginine transport were not accompanied by changes in expression of cationic amino acid transporter (CAT)-1 protein, the main L-arginine transporter in PAEC. Unlike holotoxin, the β-oligomer-binding-subunit of PTX did not affect L-arginine transport in PAEC, suggesting that Gαi ribosylation is an important step in the activation of L-arginine transport by PTX. An activator of adenylate cyclase, forskolin, and an activator of protein kinase A (PKA), Sp-cAMPS, did not affect L-arginine transport in PAEC. In addition, inhibitors of PKA or adenylate cyclase did not change the activating effect of PTX on L-arginine uptake. Long-term treatment with PTX (18 h) induced a 40% decrease in protein kinase C (PKC)-α but did not affect the activities of PKC-ε and PKC-ζ in PAEC. An activator of PKC-α, phorbol 12-myristate 13-acetate, abrogated the activation of L-arginine transport in PAEC treated with PTX. Incubation of PTX-treated PAEC with phorbol 12-myristate 13-acetate in combination with an inhibitor of PKC-α (Go 6976) restored the activating effects of PTX on L-arginine uptake, suggesting PTX-induced activation of L-arginine transport is mediated through downregulation of PKC-α. Measurements of nitric oxide (NO) production by PAEC revealed that long-term treatment with PTX induced twofold increases in the amount of NO in PAEC. PTX also increased L-[3H]citrulline production from extracellular L-[3H]arginine without affecting endothelial NO synthase activity. These results demonstrate that PTX increased NO production through activation of L-arginine transport in PAEC.
|Original language||English (US)|
|Journal||American Journal of Physiology - Lung Cellular and Molecular Physiology|
|Issue number||5 30-5|
|State||Published - May 2004|
- Cationic amino acid transporter
- G proteins
- Protein kinase C-α