Personalized and muscle-specific OXPHOS measurement with integrated CrCEST MRI and proton MR spectroscopy

Ryan R. Armbruster, Dushyant Kumar, Blake Benyard, Paul Jacobs, Aditi Khandavilli, Fang Liu, Ravi Prakash Reddy Nanga, Shana McCormack, Anne R. Cappola, Neil Wilson, Ravinder Reddy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Creatine chemical exchange saturation transfer (CrCEST) MRI is an emerging high resolution and noninvasive method for measuring muscle specific oxidative phosphorylation (OXPHOS). However, CrCEST measurements are sensitive to changes in muscle pH, which might confound the measurement and interpretation of creatine recovery time (τCr). Even with the same prescribed exercise stimulus, the extent of acidification and hence its impact on τCr is expected to vary between individuals. To address this issue, a method to measure pH pre- and post-exercise and its impact on CrCEST MRI with high temporal resolution is needed. In this work, we integrate carnosine 1H- magnetic resonance spectroscopy (MRS) and 3D CrCEST to establish “mild” and “moderate/intense” exercise stimuli. We then test the dependence of CrCEST recovery time on pH using different exercise stimuli. This comprehensive metabolic imaging protocol will enable personalized, muscle specific OXPHOS measurements in both healthy aging and myriad other disease states impacting muscle mitochondria.

Original languageEnglish (US)
Article number5387
JournalNature communications
Volume15
Issue number1
DOIs
StatePublished - Dec 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

PubMed: MeSH publication types

  • Journal Article

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