Persistently elevated level of IL-8 in chlamydia trachomatis infected HeLa 229 cells is dependent on intracellular available iron

Aruna Singh Mittal, Harsh Vardhan, Raini Dutta, Vikas Vats, Rishein Gupta, Rajneesh Jha, Hem Chandra Jha, Pragya Srivastava, Apurb Rashmi Bhengraj

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Abstract

Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.

Original languageEnglish (US)
Article number417658
JournalMediators of inflammation
Volume2009
DOIs
StatePublished - 2009

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