Permissibility of different cell types for the growth of avian metapneumovirus

A. Tiwari; Devi P. Patnayak; Yogesh Chander; Sagar M. Goyal

doi:10.1016/j.jviromet.2006.07.020

Permissibility of different cell types for the growth of avian metapneumovirus

A. Tiwari, Devi P. Patnayak, Yogesh Chander, Sagar M. Goyal

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Vero cells are commonly used for the growth of avian metapneumovirus subtype C (aMPV-C). This study was conducted to evaluate 17 different cell types for the growth of a Minnesota strain of aMPV-C. The virus was inoculated into these cell types and virus growth was monitored by the development of cytopathic effects (cpe) and immunofluorescence. Virus growth was obtained in 6 of 17 cell types tested with the highest virus titers observed in BGM and DF-1 cells. The flow cytometric analysis of cells at 72 h post inoculation found the highest number of infected cells in BGM cells followed by QT-35 cells. At 48 h post inoculation, DF-1 and BGM cells showed the highest number of infected cells. These results suggest that BGM, QT-35, and DF-1 cells can be used for high titer propagation of aMPV-C.

Original language	English (US)
Pages (from-to)	80-84
Number of pages	5
Journal	Journal of Virological Methods
Volume	138
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2006.07.020
State	Published - Dec 2006

Bibliographical note

Funding Information:
This study was conducted in part by a grant from the Minnesota Turkey Growers Association.

Keywords

Avian metapneumovirus
Cell lines

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10.1016/j.jviromet.2006.07.020

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title = "Permissibility of different cell types for the growth of avian metapneumovirus",

abstract = "Vero cells are commonly used for the growth of avian metapneumovirus subtype C (aMPV-C). This study was conducted to evaluate 17 different cell types for the growth of a Minnesota strain of aMPV-C. The virus was inoculated into these cell types and virus growth was monitored by the development of cytopathic effects (cpe) and immunofluorescence. Virus growth was obtained in 6 of 17 cell types tested with the highest virus titers observed in BGM and DF-1 cells. The flow cytometric analysis of cells at 72 h post inoculation found the highest number of infected cells in BGM cells followed by QT-35 cells. At 48 h post inoculation, DF-1 and BGM cells showed the highest number of infected cells. These results suggest that BGM, QT-35, and DF-1 cells can be used for high titer propagation of aMPV-C.",

keywords = "Avian metapneumovirus, Cell lines",

author = "A. Tiwari and Patnayak, {Devi P.} and Yogesh Chander and Goyal, {Sagar M.}",

note = "Funding Information: This study was conducted in part by a grant from the Minnesota Turkey Growers Association.",

year = "2006",

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doi = "10.1016/j.jviromet.2006.07.020",

language = "English (US)",

volume = "138",

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journal = "Journal of Virological Methods",

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T1 - Permissibility of different cell types for the growth of avian metapneumovirus

AU - Tiwari, A.

AU - Patnayak, Devi P.

AU - Chander, Yogesh

AU - Goyal, Sagar M.

N1 - Funding Information: This study was conducted in part by a grant from the Minnesota Turkey Growers Association.

PY - 2006/12

Y1 - 2006/12

N2 - Vero cells are commonly used for the growth of avian metapneumovirus subtype C (aMPV-C). This study was conducted to evaluate 17 different cell types for the growth of a Minnesota strain of aMPV-C. The virus was inoculated into these cell types and virus growth was monitored by the development of cytopathic effects (cpe) and immunofluorescence. Virus growth was obtained in 6 of 17 cell types tested with the highest virus titers observed in BGM and DF-1 cells. The flow cytometric analysis of cells at 72 h post inoculation found the highest number of infected cells in BGM cells followed by QT-35 cells. At 48 h post inoculation, DF-1 and BGM cells showed the highest number of infected cells. These results suggest that BGM, QT-35, and DF-1 cells can be used for high titer propagation of aMPV-C.

AB - Vero cells are commonly used for the growth of avian metapneumovirus subtype C (aMPV-C). This study was conducted to evaluate 17 different cell types for the growth of a Minnesota strain of aMPV-C. The virus was inoculated into these cell types and virus growth was monitored by the development of cytopathic effects (cpe) and immunofluorescence. Virus growth was obtained in 6 of 17 cell types tested with the highest virus titers observed in BGM and DF-1 cells. The flow cytometric analysis of cells at 72 h post inoculation found the highest number of infected cells in BGM cells followed by QT-35 cells. At 48 h post inoculation, DF-1 and BGM cells showed the highest number of infected cells. These results suggest that BGM, QT-35, and DF-1 cells can be used for high titer propagation of aMPV-C.

KW - Avian metapneumovirus

KW - Cell lines

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Permissibility of different cell types for the growth of avian metapneumovirus

Abstract

Bibliographical note

Keywords

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