Pericentromere tension is self-regulated by spindle structure in metaphase

Jeremy M. Chacón, Soumya Mukherjee, Breanna M. Schuster, Duncan J. Clarke, Melissa K. Gardner

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

During cell division, a mitotic spindle is built by the cell and acts to align and stretch duplicated sister chromosomes before their ultimate segregation into daughter cells. Stretching of the pericentromeric chromatin during metaphase is thought to generate a tension-based signal that promotes proper chromosome segregation. However, it is not known whether the mitotic spindle actively maintains a set point tension magnitude for properly attached sister chromosomes to facilitate robust mechanochemical checkpoint signaling. By imaging and tracking the thermal movements of pericentromeric fluorescent markers in Saccharomyces cerevisiae, we measured pericentromere stiffness and then used the stiffness measurements to quantitatively evaluate the tension generated by pericentromere stretch during metaphase in wild-type cells and in mutants with disrupted chromosome structure. We found that pericentromere tension in yeast is substantial (4-6 pN) and is tightly self-regulated by the mitotic spindle: through adjustments in spindle structure, the cell maintains wild-type tension magnitudes even when pericentromere stiffness is disrupted.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalJournal of Cell Biology
Volume205
Issue number3
DOIs
StatePublished - 2014

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