Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans

Oliver Laeyendecker; Charla Henson; Ronald H. Gray; Ruby H.N. Nguyen; Bobbi Jo Horne; Maria J. Wawer; David Serwadda; Noah Kiwanuka; Rhoda Ashley Morrow; Wayne Hogrefe; Thomas C. Quinn

doi:10.1128/JCM.42.4.1794-1796.2004

Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans

Oliver Laeyendecker
, Charla Henson
, Ronald H. Gray
, Ruby H.N. Nguyen
, Bobbi Jo Horne
, Maria J. Wawer
, David Serwadda
, Noah Kiwanuka
, Rhoda Ashley Morrow
, Wayne Hogrefe
, Thomas C. Quinn

Epidemiology & Community Health

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.

Original language	English (US)
Pages (from-to)	1794-1796
Number of pages	3
Journal	Journal of clinical microbiology
Volume	42
Issue number	4
DOIs	https://doi.org/10.1128/JCM.42.4.1794-1796.2004
State	Published - Apr 2004

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Laeyendecker, O., Henson, C., Gray, R. H., Nguyen, R. H. N., Horne, B. J., Wawer, M. J., Serwadda, D., Kiwanuka, N., Morrow, R. A., Hogrefe, W., & Quinn, T. C. (2004). Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans. Journal of clinical microbiology, 42(4), 1794-1796. https://doi.org/10.1128/JCM.42.4.1794-1796.2004

Laeyendecker, O, Henson, C, Gray, RH, Nguyen, RHN, Horne, BJ, Wawer, MJ, Serwadda, D, Kiwanuka, N, Morrow, RA, Hogrefe, W & Quinn, TC 2004, 'Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans', Journal of clinical microbiology, vol. 42, no. 4, pp. 1794-1796. https://doi.org/10.1128/JCM.42.4.1794-1796.2004

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abstract = "Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.",

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AU - Gray, Ronald H.

AU - Nguyen, Ruby H.N.

AU - Horne, Bobbi Jo

AU - Wawer, Maria J.

AU - Serwadda, David

AU - Kiwanuka, Noah

AU - Morrow, Rhoda Ashley

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AU - Quinn, Thomas C.

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Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans

Abstract

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