Percoll Process Can Improve Semen Quality and Fertility in Turkey Breeders

K. H. Choi, D. A. Emery, D. E. Straub, C. S. Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A percoll density gradient technique was developed for producing high quality turkey semen and improving the fertility by removing deleterious cellular components, including spermiophages, bacteria, abnormal or dead spermatozoa, and other cellular debris. The combination of three different percoll densities, 1.05, 1.07, and 1.08, showed the best resolution and was selected to prepare a discontinuous percoll density gradient to obtain healthy spermatozoa from semen samples. Bacteria, spermiophages, and abnormal or dead spermatozoa were detected from the density range from 1.05, 1.05 to 1.07, and 1.07 to 1.08, respectively. Healthy spermatozoa were collected from the density greater than 1.08. Spermatozoa obtained from percoll density gradient centrifugation showed better sperm motility than those from unprocessed pooled semen. Bacteria including Escherichia coli, Staphylococcus aureus, and Proteus spp., were predominant contaminants in turkey semen, and the numbers of cells were approximately 5 × 105 to 1 × 109 cfu/ml. The overall fertility rates in hens inseminated with processed percoll density gradient were higher than those in hens with unprocessed semen especially for unhealthy sperm. In conclusion, semen quality can be improved by percoll density gradient centrifugation, which augmented the fertility of turkey breeders.

Original languageEnglish (US)
Pages (from-to)702-707
Number of pages6
JournalAsian-Australasian Journal of Animal Sciences
Volume12
Issue number5
DOIs
StatePublished - Aug 1999

Keywords

  • Artificial Insemination
  • Normal or Yellow-Colored Semen
  • Percoll Density Gradient Centrifugation
  • Spermatozoa
  • Spermiophages
  • Turkey

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