Peptide:MHCII Tetramer-Based Cell Enrichment for the Study of Epitope-Specific CD4+ T Cells

Dmitri I. Kotov, Marc K. Jenkins

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Epitope-specific CD4+ T cells can be labeled in complex cell mixtures from secondary lymphoid organs with fluorophore-labeled peptide:major histocompatibility complex class II (p:MHCII) tetramers and then detected by flow cytometry. Magnetic enrichment of tetramer-bound cells before flow cytometry increases the sensitivity of detection to the point where epitope-specific cells can be studied even when very rare at early and late times after the host has been exposed to the epitope. This method is very useful for studying polyclonal epitope-specific CD4+ T cells under physiological conditions.

Original languageEnglish (US)
Article numbere75
JournalCurrent Protocols in Immunology
Volume125
Issue number1
DOIs
StatePublished - Jun 2019

Keywords

  • CD4 T cells
  • epitope specific
  • magnetic-bead enrichment
  • naive CD4 T cells

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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