Peptide coatings enhance keratinocyte attachment towards improving the peri-implant mucosal seal

Vasiliki P. Koidou, Prokopios P. Argyris, Erik P. Skoe, Juliana Mota Siqueira, Xi Chen, Lei Zhang, James E. Hinrichs, Massimo Costalonga, Conrado Aparicio

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41 Scopus citations


There is a critical need for preventing peri-implantitis as its prevalence has increased and dental implants lack features to prevent it. Research strategies to prevent peri-implantitis have focused on modifying dental implants to incorporate different antimicrobial agents. An alternative strategy consists of barring the expansion of the biofilm subgingivally by forming a long-lasting permucosal seal between the soft tissue and the implant surface. Here, we innovatively biofunctionalized titanium with bioinspired peptide coatings to strengthen biological interactions between epithelial cells and the titanium surface. We selected laminin 332- and ameloblastin-derived peptides (Lam, Ambn). Laminin 332 participates in the formation of hemidesmosomes by keratinocytes and promotes epithelial attachment around teeth; and ameloblastin, an enamel derived protein, is involved in tissue regeneration events following disruption of the periodontium. Lam, Ambn or combinations of both peptides were covalently immobilized on titanium discs. Successful immobilization of the peptides was confirmed by contact angle goniometry, X-ray photoelectron spectroscopy and fluorescent labelling of the peptides. Additionally, we confirmed the mechanical and thermochemical stability of the peptides on Ti substrates. Proliferation and hemidesmosome formation of human keratinocytes (TERT-2/OKF-6) were assessed by immunofluorescence labelling. The peptide-coated surfaces increased cell proliferation for up to 48 h in culture compared to control surfaces. Most importantly, formation of hemidesmosomes by keratinocytes was significantly increased on surfaces coated with Ambn + Lam peptides compared to control (p < 0.01) and monopeptide coatings (p < 0.005). Together, these results support the Ambn + Lam multipeptide coating as a promising candidate for inducing a permucosal seal around dental implants.

Original languageEnglish (US)
Pages (from-to)1936-1945
Number of pages10
JournalBiomaterials science
Issue number7
StatePublished - Jul 2018

Bibliographical note

Funding Information:
The authors would like to thank Professor Mark Herzberg, School of Dentistry of the University of Minnesota for allowing access to his lab facilities to perform some parts of the cell culture work. Parts of this work were carried out in the University of Minnesota I.T. Characterization Facility, which receives partial support from NSF through the MRSEC program. This research study was supported by the Erwin Schaffer Chair in the Advanced Education Program in Periodontology at the University of Minnesota and the National Institute for Dental and Craniofacial Research of the National Institutes of Health [grant number RO1DE026117]. Statistical support was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health [grant number UL1TR000114]. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The funding bodies had no role in study design, the collection, analysis and interpretation of data, in the writing of the report, and in the decision to submit the article for publication.

Publisher Copyright:
© The Royal Society of Chemistry.


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