Peptide-based inhibition of NF-κB rescues diaphragm muscle contractile dysfunction in a murine model of duchenne muscular dystrophy

Jennifer M. Peterson, William Kline, Benjamin D. Canan, Daniel J. Ricca, Brian Kaspar, Dawn A. Delfín, Kelly Dirienzo, Paula R. Clemens, Paul D. Robbins, Albert S. Baldwin, Pat Flood, Pravin Kaumaya, Michael Freitas, Joe N. Kornegay, Jerry R. Mendell, Jill A. Rafael-Fortney, Denis C. Guttridge, Paul Ml Janssen

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Deterioration of diaphragm function is one of the prominent factors that contributes to the susceptibility of serious respiratory infections and development of respiratory failure in patients with Duchenne Muscular Dystrophy (DMD). The NF-κB signaling pathway has been implicated as a contributing factor of dystrophic pathology, making it a potential therapeutic target. Previously, we demonstrated that pharmacological inhibition of NF-κB via a small NEMO Binding Domain (NBD) peptide was beneficial for reducing pathological features of mdxmice. Now, we stringently test the effectiveness and clinical potential of NBD by treating mdx mice with various formulations of NBD and use diaphragm function as our primary outcome criteria. We found that administering DMSO-soluble NBD rescued 78% of the contractile deficit between mdx and wild-type (WT) diaphragm. Interestingly, synthesis of a GLP NBD peptide as an acetate salt permitted its solubility in water, but as a negative consequence, also greatly attenuated functional efficacy. However, replacing the acetic acid counterion of the NBD peptide with trifluoroacetic acid retained the pep-tide's water solubility and significantly restored mdx diaphragm contractile function and improved histopathological indices of disease in both diaphragm and limb muscle. Together, these results support the feasibility of using a mass-produced, water- soluble NBD peptide for clinical use.

Original languageEnglish (US)
Pages (from-to)508-515
Number of pages8
JournalMolecular Medicine
Volume17
Issue number5-6
DOIs
StatePublished - May 1 2011
Externally publishedYes

Fingerprint Dive into the research topics of 'Peptide-based inhibition of NF-κB rescues diaphragm muscle contractile dysfunction in a murine model of duchenne muscular dystrophy'. Together they form a unique fingerprint.

  • Cite this

    Peterson, J. M., Kline, W., Canan, B. D., Ricca, D. J., Kaspar, B., Delfín, D. A., Dirienzo, K., Clemens, P. R., Robbins, P. D., Baldwin, A. S., Flood, P., Kaumaya, P., Freitas, M., Kornegay, J. N., Mendell, J. R., Rafael-Fortney, J. A., Guttridge, D. C., & Janssen, P. M. (2011). Peptide-based inhibition of NF-κB rescues diaphragm muscle contractile dysfunction in a murine model of duchenne muscular dystrophy. Molecular Medicine, 17(5-6), 508-515. https://doi.org/10.2119/molmed.2010.00263