Penetrating keratoplasty using 37 C organ cultured cornea

D. J. Doughman, J. E. Harris, M. K. Schmitt

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Sixty-three penetrating keratoplasties were performed in 63 patients using donor corneas stored by 37°C organ culture incubation for an average of 13 days (range 2 to 35 days). No grafts failed before 4 wk, except 1 which was stored at 4°C for 48 hr prior to transplantation, indicating that endothelial viability is maintained when proper storage conditions are maintained. Sixteen grafts have failed as a result of factors other than the storage method. This clinical study confirms the authors' previous laboratory conclusions that 37°C OC incubation up to 35 days improves the quality of donor endothelium compared with other methods of storage, and it represents a significant advance in corneal preservation.

Original languageEnglish (US)
Pages (from-to)op-778-793
JournalTransactions of the American Academy of Ophthalmology and Otolaryngology
Issue number5
StatePublished - Dec 1 1976


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