Penetrance of symbiont-mediated parthenogenesis is driven by reproductive rate in a parasitoid wasp

Amelia R.I. Lindsey, Richard Stouthamer

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Trichogramma wasps are tiny parasitoids of lepidopteran eggs, used extensively for biological control. They are often infected with the bacterial symbiont Wolbachia, which converts Trichogramma to an asexual mode of reproduction, whereby females develop from unfertilized eggs. However, this Wolbachia-induced parthenogenesis is not always complete, and previous studies have noted that infected females will produce occasional males in the lab. The conditions that reduce penetrance of the parthenogenesis phenotype are not well understood. We hypothesized that more ecologically relevant conditions of limited host access will sustain female-biased sex ratios. After restricting access to host eggs, we found a strong relationship between reproductive rate and sex ratio. By limiting reproduction to one hour a day, wasps could sustain up to 100% effective parthenogenesis for one week, with no significant impact on total fecundity. Reproductive output in the first 24-hours appears to be critical to the total sex ratio of the entire brood. Limiting oviposition in that period resulted in more effective parthenogenesis after one week, again without any significant impact on total fecundity. Our data suggest that this phenomenon may be due to the depletion of Wolbachia when oviposition occurs continuously, whereas Wolbachia titers may recover when offspring production is limited. In addition to the potential to improve mass rearing of Trichogramma for biological control, findings from this study help elucidate the context-dependent nature of a pervasive symbiotic relationship.

Original languageEnglish (US)
Article numbere3505
Pages (from-to)e3505
JournalPeerJ
Volume2017
Issue number6
DOIs
StatePublished - 2017
Externally publishedYes

Bibliographical note

Funding Information:
Wethank Barbara Baker and Christina Luu for their assistance in collecting many tiny wasps into ethanol, and Sarah Lillian for her statistical advice. We also thank Paul Rugman-Jones, Eric Smith, Matthew Daugherty, Jason Stajich, and three reviewers for helpful discussions and feedback on drafts of the manuscript. This work was supported by the National Science Foundation (DEB 1501227 to ARIL); the United States Department of Agriculture (NIFA 194617 to RS and NIFA 2016-67011-24778 to ARIL); and Robert and Peggy van den Bosch Memorial Scholarships to ARIL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2017 Lindsey and Stouthamer.

Keywords

  • Asexual
  • Reproductive modification
  • Sex ratio
  • Symbiosis
  • Trichogramma
  • Wolbachia

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