TY - JOUR
T1 - Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects
AU - Ning, Xin
AU - Liu, Ying
AU - Jia, Mengdi
AU - Wang, Qidi
AU - Sun, Ziyan
AU - Ji, Li
AU - Mayo, Kevin H.
AU - Zhou, Yifa
AU - Sun, Lin
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/6/15
Y1 - 2021/6/15
N2 - Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities. Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively. The RG-I domains were further purified and characterized. Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains. WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains. Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.
AB - Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities. Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively. The RG-I domains were further purified and characterized. Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains. WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains. Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.
KW - Antioxidant activities
KW - Pectin
KW - Radix Sophorae Tonkinensis
KW - Structural characterization
UR - https://www.scopus.com/pages/publications/85102293101
UR - https://www.scopus.com/pages/publications/85102293101#tab=citedBy
U2 - 10.1016/j.carbpol.2021.117925
DO - 10.1016/j.carbpol.2021.117925
M3 - Article
C2 - 33838804
AN - SCOPUS:85102293101
SN - 0144-8617
VL - 262
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 117925
ER -