Peak power output is maintained in rabbit psoas and rat soleus single muscle fibers when CTP replaces ATP

Philip A. Wahr, Joseph M. Metzger

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The chemomechanical coupling mechanism in striated muscle contraction was examined by changing the nucleotide substrate from ATP to CTP. Maximum shortening velocity [extrapolation to zero force from force-velocity relation (V(max)) and slope of slack test plots (V0)], maximum isometric force (P0); power, and the curvature of the force-velocity curve [α/P(o) (dimensionless parameter inversely related to the curvature)] were determined during maximum Ca2+-activated isotonic contractions of fibers from fast rabbit psoas and slow rat soleus muscles by using 0.2 mM MgATP, 4 mM MgATP, 4 mM MgCTP, or 10 mM MgCTP as the nucleotide substrate. In addition to a decrease in the maximum Ca2+-activated force in both fiber types, a change from 4 mMATP to 10 mM CTP resulted in a decrease in V(max) in psoas fibers from 3.26 to 1.87 muscle length/s. In soleus fibers, V(max), was reduced from 1.94 to 0.90 muscle length/s by this change in nucleotide. Surprisingly, peak power was unaffected in either fiber type by the change in nucleotide as the result of a three- to fourfold decrease in the curvature of the force-velocity relationship. The results are interpreted in terms of the Huxley model of muscle contraction as an increase in f1 and g1 coupled to a decrease in g2 (where f1 is the rate of cross-bridge attachment and g1 and g2 are rates of detachment) when CTP replaces ATP. This adequately accounts for the observed changes in P(o), α/P(o), and V(max). However, the two-state Huxley model does not explicitly reveal the cross-bridge transitions that determine curvature of the force-velocity relationship. We hypothesize that a nucleotide-sensitive transition among strong-binding cross-bridge states following P(i) release, but before the release of the nucleotide diphosphate, underlies the alterations in α/P(o) reported here.

Original languageEnglish (US)
Pages (from-to)76-83
Number of pages8
JournalJournal of Applied Physiology
Volume85
Issue number1
StatePublished - Jul 1 1998

Keywords

  • Adenosine 5'-triphosphate analogs
  • Mechanics
  • Muscle contraction

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