Arsenic, a class I human carcinogen, is ubiquitously found throughout the environment and around the globe, posing a great public health concern. Notably, Bangladesh and regions of West Bengal have been found to have high levels (0.5–4600 μg/L) of arsenic drinking water contamination, and approximately 50 million of the world's 200 million people chronically exposed to arsenic in Bangladesh alone. This study was carried out to examine genome-wide gene expression changes in individuals chronically exposed to arsenic-contaminated drinking water. Our study population includes twenty-nine Bangladeshi female participants with urinary arsenic levels ranging from 22.32 to 1828.12 μg/g creatinine. RNA extracted from peripheral blood mononuclear cells (PBMCs) were evaluated using RNA-Sequencing analysis. Our results indicate that a total of 1,054 genes were significantly associated with increasing urinary arsenic levels (FDR p < 0.05), which include 418 down-regulated and 636 up-regulated genes. Further Ingenuity Pathway Analysis revealed potential target genes (DAPK1, EGR2, APP), microRNAs (miR-155, -338, −210) and pathways (NOTCH signaling pathway) related to arsenic carcinogenesis. The selection of female-only participants provides a homogenous study population since arsenic has significant sex dependent effects, and the wide exposure range provides new insight for key gene expression changes that correlate with increasing urinary arsenic levels.
|Original language||English (US)|
|State||Published - Apr 2020|
Bibliographical noteFunding Information:
We thank the NYULMC Genome Technology Center, partially supported by the Cancer Center Support Grant P30CA016087 . This work was supported by grants from the US National Institutes of Health : R01ES026138 , P30ES000260 , R01ES029359 , and R01ES022935 . Appendix A
We thank the NYULMC Genome Technology Center, partially supported by the Cancer Center Support Grant P30CA016087. This work was supported by grants from the US National Institutes of Health: R01ES026138, P30ES000260, R01ES029359, and R01ES022935.