Pathologic regulation of collagen I by an aberrant protein phosphatase 2A/histone deacetylase C4/MicroRNA-29 signal axis in idiopathic pulmonary fibrosis fibroblasts

Wajahat Khalil, Hong Xia, Vidya Bodempudi, Judy Kahm, Polla Hergert, Karen Smith, Mark Peterson, Matthew Parker, Jeremy Herrera, Peter B Bitterman, Craig A Henke

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Idiopathic pulmonary fibrosis (IPF) is characterized by the relentless expansion of fibroblasts depositing type I collagen within the alveolar wall and obliterating the alveolar airspace. MicroRNA (miR)-29 is a potent regulator of collagen expression. In IPF, miR-29 levels are low, whereas type I collagen expression is high. However, the mechanism for suppression of miR-29 and increased type I collagen expression in IPF remains unclear. Here we show that when IPF fibroblasts are seeded on polymerized type I collagen, miR-29c levels are suppressed and type I collagen expression is high. In contrast, miR-29c is high and type I collagen expression is low in control fibroblasts. We demonstrate that the mechanism for suppression of miR-29 during IPF fibroblast interaction with polymerized collagen involves inappropriately low protein phosphatase (PP) 2A function, leading to histone deacetylase (HDA) C4 phosphorylation and decreased nuclear translocation of HDAC4. We demonstrate that overexpression of HDAC4 in IPF fibroblasts restored miR-29c levels and decreased type I collagen expression, whereas knocking down HDAC4 in control fibroblasts suppressed miR-29c levels and increased type I collagen expression. Our data indicate that IPF fibroblast interaction with polymerized type I collagen results in an aberrant PP2A/HDAC4 axis, which suppresses miR-29, causing a pathologic increase in type I collagen expression.

Original languageEnglish (US)
Pages (from-to)391-399
Number of pages9
JournalAmerican journal of respiratory cell and molecular biology
Volume53
Issue number3
DOIs
StatePublished - Sep 1 2015

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Protein Phosphatase 2
Idiopathic Pulmonary Fibrosis
Histone Deacetylases
Fibroblasts
Collagen Type I
MicroRNAs
Collagen
Phosphorylation

Keywords

  • HDAC4
  • IPF fibroblasts
  • PP2A
  • Type I collagen
  • miR-29

Cite this

Pathologic regulation of collagen I by an aberrant protein phosphatase 2A/histone deacetylase C4/MicroRNA-29 signal axis in idiopathic pulmonary fibrosis fibroblasts. / Khalil, Wajahat; Xia, Hong; Bodempudi, Vidya; Kahm, Judy; Hergert, Polla; Smith, Karen; Peterson, Mark; Parker, Matthew; Herrera, Jeremy; Bitterman, Peter B; Henke, Craig A.

In: American journal of respiratory cell and molecular biology, Vol. 53, No. 3, 01.09.2015, p. 391-399.

Research output: Contribution to journalArticle

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