Pathogenesis of senecavirus a infection in finishing pigs

Lok R. Joshi, Maureen H V Fernandes, Travis Clement, Steven Lawson, Angela Pillatzki, Talita P. Resende, Fabio A. Vannucci, Gerald F. Kutish, Eric A. Nelson, Diego G. Diel

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Senecavirus A (SVA) is an emerging picornavirus that has been associated with vesicular disease and neonatal mortality in swine. Many aspects of SVA infection biology and pathogenesis, however, remain unknown. Here the pathogenesis of SVA was investigated in finishing pigs. Animals were inoculated via the oronasal route with SVA strain SD15-26 and monitored for clinical signs and lesions associated with SVA infection. Viraemia was assessed in serum and virus shedding monitored in oral and nasal secretions and faeces by real-time reverse transcriptase quantitative PCR (RT-qPCR) and/or virus isolation. Additionally, viral load and tissue distribution were assessed during acute infection and following convalescence from disease. Clinical signs characterized by lethargy and lameness were first observed on day 4 post-inoculation (pi) and persisted for approximately 2-10 days. Vesicular lesions were first observed on day 4 pi on the snout and/or feet, affecting the coronary bands, dewclaws, interdigital space and heel/sole of SVA-infected animals. A short-term viraemia was observed between days 3 and 10 pi, whereas virus shedding was detected between days 1 and 28 pi in oral and nasal secretions and faeces. Notably, RT-qPCR and in situ hybridization (ISH) performed on tissues collected on day 38 pi revealed the presence of SVA RNA in the tonsils of all SVA-infected animals. Serological responses to SVA were characterized by early neutralizing antibody responses (day 5 pi), which coincided with decreased levels of viraemia, virus shedding and viral load in tissues. This study provides significant insights into the pathogenesis and infectious dynamics of SVA in swine.

Original languageEnglish (US)
Article number000631
Pages (from-to)3267-3279
Number of pages13
JournalJournal of General Virology
Issue number12
StatePublished - Dec 2016

Bibliographical note

Funding Information:
We thank our colleagues from the South Dakota Animal Disease Research and Diagnostic Laboratory (SD ADRDL) Molecular Diagnostics Section for excellent technical assistance, and the staff and students from the South Dakota State University (SDSU) Animal Resource Wing (ARW) for their invaluable help with the animal experiments. This work was supported by the Swine Health Information Center (SHIC) project number 15-192 (complete genome sequencing), and by the USDA National Institute of Food and Agriculture Hatch project SD00H517-14.

Publisher Copyright:
© 2016 The Authors.


  • SVA
  • SVV
  • Seneca Valley virus
  • Senecavirus A
  • Swine
  • Vesicular disease


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