Partial purification of antigen e from mixed stems and leaves of short ragweed plant

A. Shafiee, E. J. Staba, Yusuf J Abul-Hajj

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Abstract

Short ragweed leaves‐stems, roots, and seeds were examined for the presence of the most potent pollen allergen, antigen E. Soluble proteins were precipitated by ammonium sulfate and partially purified by a combination of dialysis and anion exchange and gel filtration chromatography. Immunodiffusion, disk electrophoresis, and skin testing of short ragweed pollen‐sensitive individuals indicated that antigen E was present in leaves‐stems portion of this plant as well as the pollen.

Original languageEnglish (US)
Pages (from-to)1654-1656
Number of pages3
JournalJournal of Pharmaceutical Sciences
Volume62
Issue number10
DOIs
StatePublished - Oct 1973

Keywords

  • Allergen distribution—antigen E in short ragweed leaves‐stems, roots, and seeds
  • Antigen E, short ragweed—distribution in leaves‐stems, roots, and seeds, isolation from leaves‐stems
  • Ragweed leaves‐stems, roots, and seeds—distribution and isolation of antigen E

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