Partial induction of the myogenic program in noncommitted adult stem cells

Fikru B Bedada, Thomas Braun

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The ability of multipotent adult stem cells (MASCs) and mesenchymal stem cells (MSCs) to acquire certain biochemical and functional properties of differentiated cells in response to various inductive cues has raised hopes to utilize such cells for therapeutic purposes. It is of particular importance to define precisely the differentiation status of induced MASCs and MSCs, and to determine the signaling pathways that control their differentiation. The identification of such signals might help to expand and enhance specific differentiation pathways to allow the formation of fully functional muscle cells from undetermined stem cells. Here, we have analyzed the ability of MASCs to contribute to the formation of muscle syncytia. We also investigated the intracellular signaling cascades that were initiated by Wnt molecules in a murine bone marrow-derived MASC population. We found that the partial recapitulation of the myogenic program depends on Wnt-mediated signaling via β-catenin and protein kinase C, but not protein kinase A (PKA). Our experiments indicate that Wnt-mediated signaling via β-catenin and protein kinase C (PKC) does not suffice to promote cell-autonomous differentiation of MASCs, but leads to acquisition of some properties of muscle cells that allow Wnt-induced cells to fuse to preexisting myotubes.

Original languageEnglish (US)
Pages (from-to)189-201
Number of pages13
JournalCells Tissues Organs
Volume188
Issue number1-2
DOIs
StatePublished - Jul 1 2008

Keywords

  • Cell and developmental biology
  • Immunohistochemistry
  • Mouse
  • Skeletal muscle
  • Stem cell biology

Fingerprint Dive into the research topics of 'Partial induction of the myogenic program in noncommitted adult stem cells'. Together they form a unique fingerprint.

Cite this