Part E: Directed Differentiation of Human Embryonic Stem Cells into Lymphocytes

Petter S. Woll, Dan S. Kaufman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lymphocytes develop in a sequential series of lineage commitment stages from lymphoid progenitor cells that, in turn, are derived from hematopoietic stem cells. The cell-fate decisions required to form mature B, T and natural killer (NK) cells remains poorly understood, especially for humans. The ability of human embryonic stem (ES) cells to differentiate into lymphocytes provides a novel model system to better define this process. Also, as lymphocytes have important clinical applications for the treatment of malignancies and infectious diseases, human ES cell-derived lymphocytes offer a new resource for novel cell-based therapies. In this protocol we describe how to generate NK cells from human ES cells in vitro, as an example of human ES cell-derived lymphocyte development.

Original languageEnglish (US)
Title of host publicationHuman Embryonic Stem Cells
Subtitle of host publicationThe Practical Handbook
PublisherJohn Wiley & Sons, Ltd
Pages287-297
Number of pages11
ISBN (Print)9780470033562
DOIs
StatePublished - Jun 15 2007

Keywords

  • Cytokines
  • Hematopoiesis
  • Human embryonic stem cell
  • Lymphocytes
  • cells N.K.
  • in vitro culture

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