As the attempt to eradicate paratuberculosis in one red deer (Cervus elaphus) farm failed, all 167 red deer of different age groups were slaughtered and examined by culture for mycobacteria, and the farm was closed down. Spleen and hepatic lymph nodes, mediastinal lymph node, ileocecal lymph node, and ileum were collected from each animal and examined (a total of 835 organs). Neither tuberculosis lesions nor pathognomic signs of paratuberculosis were detected. Among all microscopically negative for mycobacteria organs, Mycobacterium avium subsp. paratuberculosis alone was isolated from 165 organs, M. a. avium alone from 41 organs, and both pathogens from four organs. M. a. paratuberculosis alone was detected in 71 red deer, M. a. avium alone in 13 red deer and both pathogens in 18 red deer. Using standardised RFLP methods, three IS900 RFLP types B-C1, B-C16, and B-C32 were identified among 40 M. a. paratuberculosis isolates and four IS901 RFLP types N-B1, N-B3, N-B4, and P-B3 among 17 M. a. avium isolates.
Bibliographical noteFunding Information:
The authors would like to thank L. Bejcek from District Veterinary Administration (Rokycany, Czech Republic) for collecting biological material. We also wish to thank P. Hlozek and J. Kintr for the molecular analyses L. Matlova for microbiological examinations and Z. Gregorova and A. Maslanova from the Veterinary Research Institute (Brno, Czech Republic) for finding of all literature. The research was partially supported by the grants no. QLRT-2000-00879 (Brussels, EC), QD1191 (Ministry of Agriculture, Czech Republic), and GACR 206/04/2003 (Grant Agency of the Czech Republic).
- Johne's disease
- Risk assessment
- Small terrestrial vertebrates