@inproceedings{b6e2f69703a04e6c95d2fc13dda3a7e5,
title = "Parallelized microfluidic immuno capture of circulating pancreatic cells for genetic analysis and early detection of pancreatic carcinogenesis",
abstract = "We have developed and optimized a novel microfluidic platform for (he earlv detection of pancreatic carcinogenesis. The capture of circulating pancreatic cells (CPCs) was optimized with parallelized anti-epithelial cell adhesion molecule (EpCAM) and cancer-specific anti-mucin 1 (MUC1) immunocapture. Furthermore, we showed release of intact nuclei from captured cells and genetic analysis of DNA from captured cells. In clinical samples, we found CPCs in 67% of pancreatic ductal adenocarcinoma (PDAC) and 36% precancerous pancreas cyst lesion (PCLs) patients. Our data, together with mounting evidence that CPC dissemination is an early event in pancreatic carcinogenesis [1] indicates the diagnostic potential of our platform.",
keywords = "Circulating pancreatic cells, Circulating tumor cells, Early detection, Immunocapture, Mucin expression, Pancreatic cancer",
author = "Thege, {F. L.} and Santana, {S. M.} and Lannin, {T. B.} and S. Tsai and Saha, {T. N.} and Godla, {M. E.} and Pratt, {E. D.} and Rhim, {A. D.} and Kirby, {B. J.}",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "20--22",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}
