Parallel expression profiling of barley - Stem rust interactions

Ling Zhang; Claudia Castell-Miller; Stephanie Dahl; Brian Steffenson; Andris Kleinhofs

doi:10.1007/s10142-007-0069-0

Parallel expression profiling of barley - Stem rust interactions

Ling Zhang, Claudia Castell-Miller, Stephanie Dahl, Brian Steffenson, Andris Kleinhofs

Plant Pathology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformat ion of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt -MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt -MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance.

Original language	English (US)
Pages (from-to)	187-198
Number of pages	12
Journal	Functional and Integrative Genomics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/s10142-007-0069-0
State	Published - Aug 2008

Bibliographical note

Funding Information:
Acknowledgments This is Scientific Paper no. 0701-07 from the College of Agricultural, Human, and Natural Sciences Research Center, Washington State University, Pullman, WA, 99164, Project 0196. The research was supported by the National Research Initiative of the USDA Cooperative State Research, Education, and Extension Service grant number no. 2004-35301-14635.

Keywords

Barley
Disease-resistance
Expression profiling
Microarray data
Puccinia graminis f. sp. tritici
Rust resistance

Publisher link

10.1007/s10142-007-0069-0

Find It

Find It at U of M Libraries

Cite this

@article{c0a7048b58724b47b6db72baef580d88,

title = "Parallel expression profiling of barley - Stem rust interactions",

abstract = "The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformat ion of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt -MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt -MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance.",

keywords = "Barley, Disease-resistance, Expression profiling, Microarray data, Puccinia graminis f. sp. tritici, Rust resistance",

author = "Ling Zhang and Claudia Castell-Miller and Stephanie Dahl and Brian Steffenson and Andris Kleinhofs",

note = "Funding Information: Acknowledgments This is Scientific Paper no. 0701-07 from the College of Agricultural, Human, and Natural Sciences Research Center, Washington State University, Pullman, WA, 99164, Project 0196. The research was supported by the National Research Initiative of the USDA Cooperative State Research, Education, and Extension Service grant number no. 2004-35301-14635.",

year = "2008",

month = aug,

doi = "10.1007/s10142-007-0069-0",

language = "English (US)",

volume = "8",

pages = "187--198",

journal = "Functional and Integrative Genomics",

issn = "1438-793X",

publisher = "Springer Verlag",

number = "3",

}

TY - JOUR

T1 - Parallel expression profiling of barley - Stem rust interactions

AU - Zhang, Ling

AU - Castell-Miller, Claudia

AU - Dahl, Stephanie

AU - Steffenson, Brian

AU - Kleinhofs, Andris

N1 - Funding Information: Acknowledgments This is Scientific Paper no. 0701-07 from the College of Agricultural, Human, and Natural Sciences Research Center, Washington State University, Pullman, WA, 99164, Project 0196. The research was supported by the National Research Initiative of the USDA Cooperative State Research, Education, and Extension Service grant number no. 2004-35301-14635.

PY - 2008/8

Y1 - 2008/8

N2 - The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformat ion of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt -MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt -MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance.

AB - The dominant barley stem rust resistance gene Rpg1 confers resistance to many but not all pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici (Pgt). Transformat ion of Rpg1 into susceptible cultivar Golden Promise rendered the transgenic plants resistant to Pgt pathotype MCC but not to Pgt pathotype QCC. Our objective was to identify genes that are induced/repressed during the early stages of pathogen infection to elucidate the molecular mechanisms and role of Rpg1 in defense. A messenger ribonucleic acid expression analysis using the 22K Barley1 GeneChip was conducted in all pair-wise combinations of two isolines (cv. Golden Promise and Rpg1 transgenic line G02-448F-3R) and two Pgt pathotypes (MCC and QCC) across six time points. Analysis showed that a total of 34 probe sets exhibited expression pattern differences between Golden Promise (susceptible) and G02-448F-3R (resistant) infected with Pgt -MCC. A total of 14 probe sets exhibited expression pattern differences between Pgt -MCC (avirulent) and Pgt-QCC (virulent) inoculated onto G02-448F-3R. These differentially expressed genes were activated during the early infection process, before the hypersensitive response or fungal growth inhibition occurred. Our analysis provides a list of candidate signaling components, which can be analyzed for function in Rpg1-mediated disease resistance.

KW - Barley

KW - Disease-resistance

KW - Expression profiling

KW - Microarray data

KW - Puccinia graminis f. sp. tritici

KW - Rust resistance

UR - http://www.scopus.com/inward/record.url?scp=45449101902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45449101902&partnerID=8YFLogxK

U2 - 10.1007/s10142-007-0069-0

DO - 10.1007/s10142-007-0069-0

M3 - Article

C2 - 18196301

AN - SCOPUS:45449101902

SN - 1438-793X

VL - 8

SP - 187

EP - 198

JO - Functional and Integrative Genomics

JF - Functional and Integrative Genomics

IS - 3

ER -

Parallel expression profiling of barley - Stem rust interactions

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this