Pap1-dependent regulation of the GSTII gene from the fission yeast

Chang Jin Lim, Young Wook Cho, Jae Hoon Sa, Hye Won Lim, Hong Gyum Kim, Su Jung Kim, Eun Hee Park

Research output: Contribution to journalArticle

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Abstract

The genomic DNA encoding a second glutathione S-transferase (GSTII) was previously isolated from the fission yeast Schizosaccharomyces pombe. Its expression was shown to be induced by menadione, mercuric chloride, o-dinitrobenzene, and NO-generating S-nitroso-N-acetylpenicillamine using the GSTII-lacZ fusion harboring the 910 bp upstream region from the translational initiation point. In this study, the additional fusion plasmids pGST50-590 and pGST50-6R-590 were constructed to carry the 590 bp upstream region in the vectors YEp357 and YEp367R, respectively. The synthesis of β-galactosidase from the fusion plasmid pGST50-590 was about 3-fold higher than that from the fusion plasmid pGST50-F, indicating the presence of negatively activating sequence in the -910 ∼ -590 region. It was also enhanced by the same agents, which induced the synthesis of β-galactosidase from the fusion plasmid pGST50-F. The synthesis of β-galactosidase from both fusion plasmids pGST50-F and pGST50-590 was enhanced by the overexpressed Pap1 protein. The synthesis of β-galactosidase from the two YEp367R derivatives pGST50-6R-F and pGST50-6R-590 was greatly decreased in the Pap1-negative strain TP108-3C. These results propose the Pap1-dependent regulation of the GSTII gene from the fission yeast.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalMolecules and cells
Volume14
Issue number3
StatePublished - Dec 1 2002

Keywords

  • Fission Yeast
  • Glutathione S-Transferase
  • Oxidative Stress
  • Pap1
  • Regulation
  • Schizosaccharomyces pombe

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    Lim, C. J., Cho, Y. W., Sa, J. H., Lim, H. W., Kim, H. G., Kim, S. J., & Park, E. H. (2002). Pap1-dependent regulation of the GSTII gene from the fission yeast. Molecules and cells, 14(3), 431-436.