Pain and activity measurements

David H.H. Molstad, Elizabeth W. Bradley

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Musculoskeletal pain contributes significantly to chronic pain experienced by adults and to health care use. This chapter details several methods to evaluate pain and physical activity in mice that can be applied to preclinical orthopedic models. These methods include the von Frey filament assay that measures mechanical allodynia, open-field activity assays for evaluation of ambulation, and incapacitance measurements to determine static weight bearing.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
This work was made possible by training and research grants from the National Institutes of Health (AR065397 and AR072634) and the University of Minnesota Stem Cell Institute and Board of Regents. These contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.


  • Evoked pain
  • Functional assays
  • Incapacitance
  • Mechanical allodynia
  • Open-field activity assays
  • Static weight bearing
  • von Frey
  • Extremities/physiology
  • Musculoskeletal Pain
  • Pain Measurement/methods
  • Hyperalgesia
  • Behavior, Animal
  • Animals
  • Pain Threshold
  • Weight-Bearing
  • Mice
  • Disease Models, Animal

PubMed: MeSH publication types

  • Research Support, Non-U.S. Gov't
  • Journal Article
  • Research Support, N.I.H., Extramural


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