Packaging of heterologous RNAs by a minimal bovine leukemia virus RNA packaging signal into virus particles

N. A. Jewell, L. M. Mansky

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A minimal bovine leukemia virus (BLV) RNA packaging sequence (E) required for heterologous RNAs to be packaged into BLV particles was analyzed. The BLV E was inserted into a non-viral vector, pLacZ, in order to determine if packaging of the non-viral vector RNA would occur. The construct was transfected into cells chronically infected with BLV in order to produce virus particles. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of viral RNA from virus particles revealed that non-viral RNA containing the BLV E was packaged into BLV particles, indicating that the BLV E is necessary and sufficient to allow for packaging of a non-viral vector RNA. We also analyzed the ability of a chimeric murine leukemia virus (MLV) retroviral vector (pLN) containing BLV E to be packaged into BLV particles. Interestingly, it was found that pLNA (which does not possess ψ+) could be packaged into BLV particles. This indicates that a MLV RNA region outside of ψ+ allows for packaging of the MLV RNA into BLV particles.

Original languageEnglish (US)
Pages (from-to)1161-1173
Number of pages13
JournalArchives of Virology
Volume150
Issue number6
DOIs
StatePublished - Jun 2005

Fingerprint Dive into the research topics of 'Packaging of heterologous RNAs by a minimal bovine leukemia virus RNA packaging signal into virus particles'. Together they form a unique fingerprint.

Cite this