p53-dependent inhibition of FKHRL1 in response to DNA damage through protein kinase SGK1

Han You, Ying Ju Jang, Annick Itie You-Ten, Hitoshi Okada, Jennifer Liepa, Andrew Wakeham, Kathrin Zaugg, Tak W. Mak

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

FKHRL1 (FOXO3a) and p53 are two potent stress-response regulators. Here we show that these two transcription factors exhibit "crosstalk" in vivo. In response to DNA damage, p53 activation led to FKHRL1 phosphorylation and subcellular localization change, which resulted in inhibition of FKHRL1 transcription activity. AKT was dispensable for p53-dependent suppression of FKHRL1. By contrast, serum- and glucocorticoid-inducible kinase 1 (SGK1) was significantly induced in a p53-dependent manner after DNA damage, and this induction was through extracellular signal-regulated kinase 1/2-mediated posttranslational regulation. Furthermore, inhibition of SGK1 expression by a small interfering RNA knockdown experiment significantly decreased FKHRL1 phosphorylation in response to DNA damage. Taken together, our observations reveal previously unrecognized crosstalk between p53 and FKHRL1. Moreover, our findings suggest a new pathway for understanding aging and the age dependency of human diseases governed by these two transcription factors.

Original languageEnglish (US)
Pages (from-to)14057-14062
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number39
DOIs
StatePublished - Sep 28 2004

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