p16CDKN2A expression during rat tongue carcinogenesis induced by 4-Nitroquinoline-1-oxide

Yun Hong, Chunyang Li, Juan Xia, Nelson L. Rhodus, Bin Cheng

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

p16CDKN2A is one of the most important tumor-suppressor genes and has been investigated widely in recent years for its role in oral carcinogenesis, but few have explored the relationship between its RNA and protein, especially in precancerous tissues. The aim of this study was to explore the relationship of mRNA and protein level of p16CDKN2A in rat tongue carcinogenesis process induced by 4-nitroquinoline-1-oxide. By the use of semi-quantitative RT-PCR, immunohistochemistry (IHC) and Western Blot, histologically normal, premalignant and invasive squamous cell carcinoma samples from the animal model were explored respectively. The results showed the levels of mRNA of p16CDKN2A did not significantly change during the carcinogenesis process when compared with controls. However, detectable level of P16 protein expression was lost in both the dysplasia and carcinoma groups. We could conclude that p16CDKN2A in 4NQO-induced rat tongue carcinogenesis might be inactivated predominantly by posttranscriptional regulation.

Original languageEnglish (US)
Pages (from-to)640-644
Number of pages5
JournalOral Oncology
Volume45
Issue number7
DOIs
StatePublished - Jul 2009

Bibliographical note

Funding Information:
This work was supported by the grant from the Department of Sciences and Technology, Guangdong Province, PR China (2003C32705).

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Keywords

  • 4-Nitroquinoline-1-oxide
  • Oral squamous cell carcinoma
  • Posttranscriptional mechanism
  • Rat
  • Tongue carcinoma
  • p16

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