P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells

N. H. Kim; Y. H. Hwang; K. S. Im; J. H. Kim; S. K. Chon; H. Y. Kim; J. H. Sur

doi:10.1016/j.rvsc.2012.04.004

P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells

N. H. Kim
, Y. H. Hwang
, K. S. Im
, J. H. Kim
, S. K. Chon
, H. Y. Kim
, J. H. Sur

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.

Original language	English (US)
Pages (from-to)	1346-1352
Number of pages	7
Journal	Research in veterinary science
Volume	93
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2012.04.004
State	Published - Dec 2012
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Ms. R.H. Jang for excellent technical assistance. Dr. Hwang contributed equally to the study. This report summarises work contained within a thesis submitted by Hwang to fulfil the requirements for an M.S. This study was supported by a grant from the Konkuk University Research Foundation 2011.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Canine
Mammary gland tumours
P-glycoprotein

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10.1016/j.rvsc.2012.04.004

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title = "P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells",

abstract = "P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5\%) and 21 malignant (63.6\%), while epithelial localised tumours were 12 cases, all malignant (36.5\%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.",

keywords = "Canine, Mammary gland tumours, P-glycoprotein",

author = "Kim, \{N. H.\} and Hwang, \{Y. H.\} and Im, \{K. S.\} and Kim, \{J. H.\} and Chon, \{S. K.\} and Kim, \{H. Y.\} and Sur, \{J. H.\}",

note = "Funding Information: The authors thank Ms. R.H. Jang for excellent technical assistance. Dr. Hwang contributed equally to the study. This report summarises work contained within a thesis submitted by Hwang to fulfil the requirements for an M.S. This study was supported by a grant from the Konkuk University Research Foundation 2011.",

year = "2012",

month = dec,

doi = "10.1016/j.rvsc.2012.04.004",

language = "English (US)",

volume = "93",

pages = "1346--1352",

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AU - Im, K. S.

AU - Kim, J. H.

AU - Chon, S. K.

AU - Kim, H. Y.

AU - Sur, J. H.

N1 - Funding Information: The authors thank Ms. R.H. Jang for excellent technical assistance. Dr. Hwang contributed equally to the study. This report summarises work contained within a thesis submitted by Hwang to fulfil the requirements for an M.S. This study was supported by a grant from the Konkuk University Research Foundation 2011.

PY - 2012/12

Y1 - 2012/12

N2 - P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.

AB - P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.

KW - Canine

KW - Mammary gland tumours

KW - P-glycoprotein

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P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells

Abstract

Bibliographical note

UN SDGs

Keywords

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