Abstract
P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1346-1352 |
| Number of pages | 7 |
| Journal | Research in veterinary science |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2012 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors thank Ms. R.H. Jang for excellent technical assistance. Dr. Hwang contributed equally to the study. This report summarises work contained within a thesis submitted by Hwang to fulfil the requirements for an M.S. This study was supported by a grant from the Konkuk University Research Foundation 2011.
Keywords
- Canine
- Mammary gland tumours
- P-glycoprotein
