P-glycoprotein expression in canine mammary gland tumours related with myoepithelial cells

N. H. Kim, Y. H. Hwang, K. S. Im, J. H. Kim, S. K. Chon, H. Y. Kim, J. H. Sur

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Abstract

P-glycoprotein is influential in chemotherapy-resistance in numerous cancers and has been widely studied in human breast cancer research, but is less studied in canine mammary gland tumour (MGT). The study was to evaluate P-glycoprotein expression and its localisations related with prognostic factors with monoclonal antibody C219, by immunohistochemistry (IHC) of 68 cases of canine malignant (n= 54) and benign (n= 14) MGT. Additional immunofluorescence (IF) and reverse transcriptase-polymerase chain reaction (RT-PCR) were also performed. There was a novel finding that P-glycoprotein expression with C219 localised at two different cell types: epithelial and myoepithelial cells. Myoepithelial localised tumours were 5 benign (35.5%) and 21 malignant (63.6%), while epithelial localised tumours were 12 cases, all malignant (36.5%). Unlike conventional belief, semi-quantitative evaluation of IHC intensity scores of C219 expression in malignant MGT was related with favourable histopathological parameters.

Original languageEnglish (US)
Pages (from-to)1346-1352
Number of pages7
JournalResearch in veterinary science
Volume93
Issue number3
DOIs
StatePublished - Dec 1 2012

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Keywords

  • Canine
  • Mammary gland tumours
  • P-glycoprotein

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