Pâ̂†TubHA4C, a new versatile vector for constitutive expression in Drosophila

Yan Zhang, Stephanie Arcia, Barbara Perez, Pedro Fernandez-Funez, Diego E. Rincon-Limas

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Several vectors for gene expression are available in Drosophila, a hub for genetics and genomics innovation. However, the vectors for ubiquitous expression have a complex structure, including coding exons, that makes in-frame cloning of cDNAs very complicated. In this report we describe a new Drosophila expression vector (pâ̂†TubHA4C) for ubiquitous expression of coding sequences under the control of a minimal 0.9 kb promoter of α1 tubulin (α1t). This plasmid was designed to include optimized multiple cloning sites (polylinker) to provide flexibility in cloning strategies. We also added the option of double labeling the expressed proteins with two C-terminal tags, the viral epitope hemagglutinin and a synthetic tetracysteine (4C) tag that binds small fluorescent compounds. This dual tag allows both in situ and biochemical detection of the desired protein. In particular, the new 4C tag technology combines easy fluorescent labeling with small arsenical compounds in live or fixed cells and tissues, while producing minimal alterations to the tagged protein due to its small size. To demonstrate the potent and ubiquitous expression under the control of the â̂†Tub promoter, bacterial lacZ was expressed and monitored in cell culture and transgenic flies. We found that the modified 0.9 kb ΔTub promoter induced similar expression levels to the intact 2.6 kb α1t promoter, supporting the inclusion of all critical regulatory elements in the new and flexible â̂† TubHA4C vector.

Original languageEnglish (US)
Pages (from-to)5407-5415
Number of pages9
JournalMolecular Biology Reports
Issue number9
StatePublished - Sep 2013


  • Drosophila
  • Hemagglutinin
  • Tetracysteine
  • Tubulin promoter
  • Ubiquitous expression
  • Vector


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