OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17: K+: H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis

James R. Johnson, Thomas A. Russo, Sarah M. Drawz, Connie Clabots, Ruth Olson, Michael A. Kuskowski, Henry Rosen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The oxidative stress response regulator OxyR was assessed as both a urinary and extra-urinary virulence factor in Escherichia coli strain UCB34 (O17:K+:H18), a representative of the emergent Clonal Group A (CGA). Compared to UCB34, the isogenic oxyR mutant exhibited increased H2O2 sensitivity, indistinguishable invitro growth, and attenuated virulence in rodent models of urinary tract, subcutaneous infection, and systemic sepsis. Complemented mutants showed virulence levels comparable to parent strains in all models. These findings uniquely fulfill molecular Koch's postulates for a putative virulence factor of CGA, provide experimental evidence of an extra-urinary virulence promoting trait in CGA, and document a role for OxyR in local and systemic extra-urinary E.coli infections.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalMicrobial Pathogenesis
Volume64
DOIs
StatePublished - Nov 1 2013

Keywords

  • Clonal Group A
  • Escherichia coli infections
  • Extra-urinary infections
  • OxyR
  • Urinary tract infections
  • Virulence

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