Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles

Amani L. Lee, Clifford T. Gee, Bradley P. Weegman, Samuel A. Einstein, Adam R. Juelfs, Hattie L. Ring, Katie R. Hurley, Sam M. Egger, Garrett Swindlehurst, Michael Garwood, William C.K. Pomerantz, Christy L. Haynes

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Oxygen homeostasis is important in the regulation of biological function. Disease progression can be monitored by measuring oxygen levels, thus producing information for the design of therapeutic treatments. Noninvasive measurements of tissue oxygenation require the development of tools with minimal adverse effects and facile detection of features of interest. Fluorine magnetic resonance imaging ( 19 F MRI) exploits the intrinsic properties of perfluorocarbon (PFC) liquids for anatomical imaging, cell tracking, and oxygen sensing. However, the highly hydrophobic and lipophobic properties of perfluorocarbons require the formation of emulsions for biological studies, though stabilizing these emulsions has been challenging. To enhance the stability and biological loading of perfluorocarbons, one option is to incorporate perfluorocarbon liquids into the internal space of biocompatible mesoporous silica nanoparticles. Here, we developed perfluorocarbon-loaded ultraporous mesostructured silica nanoparticles (PERFUMNs) as 19 F MRI detectable oxygen-sensing probes. Ultraporous mesostructured silica nanoparticles (UMNs) have large internal cavities (average = 1.8 cm 3 g -1 ), facilitating an average 17% loading efficiency of PFCs, meeting the threshold fluorine concentrations needed for imaging studies. Perfluoro-15-crown-5-ether PERFUMNs have the highest equivalent nuclei per PFC molecule and a spin-lattice (T 1 ) relaxation-based oxygen sensitivity of 0.0032 mmHg -1 s -1 at 16.4 T. The option of loading PFCs after synthesizing UMNs, rather than traditional in situ core-shell syntheses, allows for use of a broad range of PFC liquids from a single material. The biocompatible and tunable chemistry of UMNs combined with the intrinsic properties of PFCs makes PERFUMNs a MRI sensor with potential for anatomical imaging, cell tracking, and metabolic spectroscopy with improved stability.

Original languageEnglish (US)
Pages (from-to)5623-5632
Number of pages10
JournalACS Nano
Volume11
Issue number6
DOIs
StatePublished - Jun 27 2017

Fingerprint

Fluorocarbons
Silicon Dioxide
Silica
Oxygen
Nanoparticles
silicon dioxide
nanoparticles
oxygen
Magnetic resonance imaging
Imaging techniques
Fluorine
emulsions
Emulsions
fluorine
Liquids
liquids
homeostasis
Oxygenation
oxygenation
Magnetic resonance

Keywords

  • magnetic resonance
  • mesoporous silica nanoparticles
  • oximetry
  • perfluorocarbons
  • relaxometry
  • thermometry

How much support was provided by MRSEC?

  • Shared

Reporting period for MRSEC

  • Period 4

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles. / Lee, Amani L.; Gee, Clifford T.; Weegman, Bradley P.; Einstein, Samuel A.; Juelfs, Adam R.; Ring, Hattie L.; Hurley, Katie R.; Egger, Sam M.; Swindlehurst, Garrett; Garwood, Michael; Pomerantz, William C.K.; Haynes, Christy L.

In: ACS Nano, Vol. 11, No. 6, 27.06.2017, p. 5623-5632.

Research output: Contribution to journalArticle

Lee, AL, Gee, CT, Weegman, BP, Einstein, SA, Juelfs, AR, Ring, HL, Hurley, KR, Egger, SM, Swindlehurst, G, Garwood, M, Pomerantz, WCK & Haynes, CL 2017, 'Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles', ACS Nano, vol. 11, no. 6, pp. 5623-5632. https://doi.org/10.1021/acsnano.7b01006
Lee, Amani L. ; Gee, Clifford T. ; Weegman, Bradley P. ; Einstein, Samuel A. ; Juelfs, Adam R. ; Ring, Hattie L. ; Hurley, Katie R. ; Egger, Sam M. ; Swindlehurst, Garrett ; Garwood, Michael ; Pomerantz, William C.K. ; Haynes, Christy L. / Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles. In: ACS Nano. 2017 ; Vol. 11, No. 6. pp. 5623-5632.
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