Estresse oxidativo não está associadoà disfunção vascular em um modelo de diabetes induzida por aloxana em ratos

Translated title of the contribution: Oxidative stress is not associated with vascular dysfunction in a model of alloxan-induced diabetic rats

Verena Kise Capellini, Caroline Floreoto Baldo, Andréa Carla Celotto, Marcelo Eduardo Batalhão, Evelin Capellari Cárnio, Alfredo José Rodrigues, Paulo Roberto Barbosa Evora

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Objectives: To verify if an experimental model of alloxan-diabetic rats promotes oxidative stress, reduces nitric oxide bioavailability and causes vascular dysfunction, and to evaluate the effect of N-acetylcysteine (NAC) on these parameters. Methods: Alloxan-diabetic rats were treated or not with NAC for four weeks. Plasmatic levels of malondialdehyde (MDA) and nitrite/nitrate (NOx), the endothelial and inducible nitric oxide synthase (eNOS and iNOS) immunostaining and the vascular reactivity of aorta were compared among diabetic (D), treated diabetic (TD) and control (C) rats. Results: MDA levels increased in D and TD. NOx levels did not differ among groups. Endothelial eNOS immunostaining reduced and adventitial iNOS increased in D and TD. The responsiveness of rings to acetylcholine, sodium nitroprusside, and phenylephrine did not differ among groups. Conclusions: NAC had no effect on the evaluated parameters and this experimental model did not promote vascular dysfunction despite the development of oxidative stress.

Translated title of the contributionOxidative stress is not associated with vascular dysfunction in a model of alloxan-induced diabetic rats
Original languagePortuguese
Pages (from-to)530-539
Number of pages10
JournalArquivos Brasileiros de Endocrinologia e Metabologia
Volume54
Issue number6
DOIs
StatePublished - 2010

Keywords

  • Diabetes
  • N-acetylcysteine
  • Nitric oxide
  • Oxidative stress
  • Vascular function

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