Oxidative stress-induced senescence markedly increases disc cell bioenergetics

Prashanti Patil, Micol Falabella, Amal Saeed, Dayeong Lee, Brett Kaufman, Sruti Shiva, Claudette St Croix, Ben Van Houten, Laura J. Niedernhofer, Paul D. Robbins, Joon Lee, Sowa Gwendolyn, Nam V. Vo

Research output: Contribution to journalArticle

Abstract

Cellular senescence is a phenotype characterized by irreversible growth arrest, chronic elevated secretion of proinflammatory cytokines and matrix proteases, a phenomenon known as senescence-associated secretory phenotype (SASP). Biomarkers of cellular senescence have been shown to increase with age and degeneration of human disc tissue. Senescent disc cells in culture recapitulate features associated with age-related disc degeneration, including increased secretion of proinflammatory cytokines, matrix proteases, and fragmentation of matrix proteins. However, little is known of the metabolic changes that underlie the senescent phenotype of disc cells. To assess the metabolic changes, we performed a bioenergetic analysis of in vitro oxidative stress-induced senescent (SIS)human disc cells. SIS disc cells acquire SASP and exhibit significantly elevated mitochondrial content and mitochondrial ATP-linked respiration. The metabolic changes appear to be driven by the upregulated protein secretion in SIS cells as abrogation of protein synthesis using cycloheximide decreased mitochondrial ATP-linked respiration. Taken together, the results of the study suggest that the increased energy generation state supports the secretion of senescent associated proteins in SIS disc cells.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalMechanisms of Ageing and Development
Volume180
DOIs
StatePublished - Jun 2019

Fingerprint

Energy Metabolism
Cell Aging
Oxidative Stress
Phenotype
Intervertebral Disc Degeneration
Respiration
Peptide Hydrolases
Adenosine Triphosphate
Cytokines
Proteins
Cycloheximide
Heat-Shock Proteins
Cell Culture Techniques
Biomarkers
Growth

Keywords

  • Aging
  • Bioenergetics
  • Cellular senescence
  • Intervertebral disc degeneration
  • Matrix homeostasis
  • Mitochondria

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

Patil, P., Falabella, M., Saeed, A., Lee, D., Kaufman, B., Shiva, S., ... Vo, N. V. (2019). Oxidative stress-induced senescence markedly increases disc cell bioenergetics. Mechanisms of Ageing and Development, 180, 97-106. https://doi.org/10.1016/j.mad.2019.04.006

Oxidative stress-induced senescence markedly increases disc cell bioenergetics. / Patil, Prashanti; Falabella, Micol; Saeed, Amal; Lee, Dayeong; Kaufman, Brett; Shiva, Sruti; Croix, Claudette St; Van Houten, Ben; Niedernhofer, Laura J.; Robbins, Paul D.; Lee, Joon; Gwendolyn, Sowa; Vo, Nam V.

In: Mechanisms of Ageing and Development, Vol. 180, 06.2019, p. 97-106.

Research output: Contribution to journalArticle

Patil, P, Falabella, M, Saeed, A, Lee, D, Kaufman, B, Shiva, S, Croix, CS, Van Houten, B, Niedernhofer, LJ, Robbins, PD, Lee, J, Gwendolyn, S & Vo, NV 2019, 'Oxidative stress-induced senescence markedly increases disc cell bioenergetics', Mechanisms of Ageing and Development, vol. 180, pp. 97-106. https://doi.org/10.1016/j.mad.2019.04.006
Patil, Prashanti ; Falabella, Micol ; Saeed, Amal ; Lee, Dayeong ; Kaufman, Brett ; Shiva, Sruti ; Croix, Claudette St ; Van Houten, Ben ; Niedernhofer, Laura J. ; Robbins, Paul D. ; Lee, Joon ; Gwendolyn, Sowa ; Vo, Nam V. / Oxidative stress-induced senescence markedly increases disc cell bioenergetics. In: Mechanisms of Ageing and Development. 2019 ; Vol. 180. pp. 97-106.
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