OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN

J. M. Milstein; J. G. White; Kenneth F Swaiman

doi:10.1111/j.1471-4159.1968.tb11627.x

OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN

J. M. Milstein, J. G. White, Kenneth F Swaiman

Neurology

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33 Scopus citations

Abstract

Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation.

Original language	English (US)
Pages (from-to)	411-415
Number of pages	5
Journal	Journal of Neurochemistry
Volume	15
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-4159.1968.tb11627.x
State	Published - May 1968

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title = "OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN",

abstract = "Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation.",

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N2 - Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation.

AB - Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation.

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OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN

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