OXIDATIVE PHOSPHORYLATION IN MITOCHONDRIA OF DEVELOPING RAT BRAIN

J. M. Milstein, J. G. White, Kenneth F Swaiman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Abstract— Oxygen uptake, ADP/O ratios and respiratory control ratios (RCR) were studied by oxygen electrode techniques in mitochondria prepared from developing rat brain. Oxygen consumption, ADP/O ratios and RCR based on mitochondrial protein concentration increased with maturation. Of the substrates employed, succinate supported oxygen uptake best and malate poorest. The addition of exogenous NAD to the mitochondrial preparation had no effect on rate of oxygen uptake. Lack of change in ADP/O ratio in the presence of glucose, a tricarboxylic acid cycle intermediate (α‐oxoglutarate), and ATP leads us to believe that there is no significant hexokinase activity in this preparation.

Original languageEnglish (US)
Pages (from-to)411-415
Number of pages5
JournalJournal of Neurochemistry
Volume15
Issue number5
DOIs
StatePublished - May 1968

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