Oxidation of oxyphenbutazone by sheep vesicular gland microsomes and lipoxygenase

Philip S. Portoghese, Kerstin Svanborg, Bengt Samuelsson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Oxyphenbutazone was oxidized when incubated with acetone powder prepared from sheep vesicular gland microsomes or with lipoxygenase at pH 4 or 5. Oxidation also occurred at pH 8 or 9, if arachidonate or linoleate was added to either of the incubation mixtures. The oxygenated product was found to be identical with 4-hydroxyoxyphenbutazone, which was synthesized and analyzed by gas liquid chromatography and mass spectrometry. The oxygenated compound was not an inhibitor of prostaglandin biosynthesis.

Original languageEnglish (US)
Pages (from-to)748-755
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume63
Issue number3
DOIs
StatePublished - Apr 7 1975

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Oxyphenbutazone
Prostaglandin Antagonists
Lipoxygenase
Biosynthesis
Liquid chromatography
Linoleic Acid
Microsomes
Acetone
Gas chromatography
Powders
Mass spectrometry
Sheep
Oxidation
Gas Chromatography-Mass Spectrometry

Cite this

Oxidation of oxyphenbutazone by sheep vesicular gland microsomes and lipoxygenase. / Portoghese, Philip S.; Svanborg, Kerstin; Samuelsson, Bengt.

In: Biochemical and Biophysical Research Communications, Vol. 63, No. 3, 07.04.1975, p. 748-755.

Research output: Contribution to journalArticle

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