Oxidation of oxyphenbutazone by sheep vesicular gland microsomes and lipoxygenase

Philip S. Portoghese, Kerstin Svanborg, Bengt Samuelsson

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Oxyphenbutazone was oxidized when incubated with acetone powder prepared from sheep vesicular gland microsomes or with lipoxygenase at pH 4 or 5. Oxidation also occurred at pH 8 or 9, if arachidonate or linoleate was added to either of the incubation mixtures. The oxygenated product was found to be identical with 4-hydroxyoxyphenbutazone, which was synthesized and analyzed by gas liquid chromatography and mass spectrometry. The oxygenated compound was not an inhibitor of prostaglandin biosynthesis.

Original languageEnglish (US)
Pages (from-to)748-755
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Apr 7 1975

Bibliographical note

Funding Information:
A. Pfeiffer Memorial Research Fellowship.


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