TY - JOUR
T1 - Overview of mRNA expression profiling using microarrays.
AU - Katagiri, Fumiaki
AU - Glazebrook, Jane
PY - 2004/9
Y1 - 2004/9
N2 - Microarray technology allows simultaneous measurement of the mRNA levels of thousands of genes. This powerful technology has applications in addressing many biological questions that were not approachable previously; however, the enormous size of microarray data sets leads to issues of experimental design and statistical analysis that are unfamiliar to many molecular biologists. The type of array used, design of the biological experiment, number of experimental replicates, and statistical method for data analysis should all be chosen based on the scientific goals of the investigator. This overview presents a discussion of the relative merits and limitations of various methods with respect to some common applications of microarray experiments.
AB - Microarray technology allows simultaneous measurement of the mRNA levels of thousands of genes. This powerful technology has applications in addressing many biological questions that were not approachable previously; however, the enormous size of microarray data sets leads to issues of experimental design and statistical analysis that are unfamiliar to many molecular biologists. The type of array used, design of the biological experiment, number of experimental replicates, and statistical method for data analysis should all be chosen based on the scientific goals of the investigator. This overview presents a discussion of the relative merits and limitations of various methods with respect to some common applications of microarray experiments.
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U2 - 10.1002/0471142727.mb2204s67
DO - 10.1002/0471142727.mb2204s67
M3 - Article
C2 - 18265348
AN - SCOPUS:39749138021
SN - 1934-3639
VL - Chapter 22
SP - Unit 22.4
JO - Current Protocols in Molecular Biology
JF - Current Protocols in Molecular Biology
ER -